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李丹
发表时间:2014-01-03 阅读次数:8544次

李丹  博士/副教授/硕导


联系方式:

        复旦大学环境科学与工程系

        上海市杨浦区邯郸路220号

        办公室:环境科学与工程系133房间

        邮政编码:200433

        电子邮箱:lidanfudan@fudan.edu.cn

 

工作经历:

2013.12~至今     复旦大学 环境科学与工程系

2011.11~2013.11  美国麻省理工学院(MIT)生物化学系  Postdoctoral research fellow

2010.07~2012.06  清华大学环境学院 博士后

2011.06~2011.10  美国麻省理工学院(MIT)生物化学系和东北大学 Visiting Scholar

 

教育经历:

2004.09-2010.07: 清华大学    环境科学与工程系        博士研究生

2008.10-2009.10: 美国University of California Irvine 

                Civil and Environmental Engineering      联合培养博士生

2000.09-2004.07: 山东大学    环境科学与工程学院      本科生

 

研究方向:

  • 环境污染物生物毒性作用机制及评价方法;
  • 环境中耐药菌及抗性基因形成及传播机制;
  • 环境病原微生物分子生物学检、控制技术及风险评估;

 

科研项目:

[1]自然科学基金委重大研究计培育项目项目(2017~2019):大气细颗粒物毒性组分对呼吸道病原菌耐药性的影响机制研究(No. 91643106), 项目负责人。
[2]国家自然科学基金面上项目(2015~2018):水环境中新兴污染物类抗生素效应(Like-Antibiotic Effects, L-AE)作用机制研究(No. 21477024),项目负责人。
[3]国家自然科学基金重大仪器专项(2016 ~ 2021):大气颗粒物关键化学组分与生物毒性在线分析联用系统研制(No. 21527814),子课题负责人。
[4]国家自然科学基金面上项目(2012~2015):再生水系统中VBNC (Viable but nonculturable)病原菌复活机制与控制方法研究(No. 51178242),项目负责人。
[5]科技部国家重大仪器专项(2012~2017):有毒污染物多指标快速检测仪器(,20121848339),在研,主要参加人。
[6]国家重点实验室开放基金(2014 ~ 2016):基于人源细胞应激响应途径的环境污染物毒性评价方法研究,项目负责人。
[7]复旦大学引进人才科研启动经费,2013,基于基于毒理基因组学的环境新兴污染物遗传毒性效应分析方法建立及应用研究, 项目负责人。
[8]国家863计划项目(2014~2017):水质安全生物预警监测技术与系统(No. 2014AA06A506),主要参加人。
[9]国家863计划项目(2009~2011):重大环境污染事件特征污染物检测系统与技术集成,主要参加人,已完成。
[10]国家重大水专项项目(No. 2009ZX07313-007,2009~2011):城市水环境安全监管体系研究与综合示范,主要参加人,已完成。
[11]环境模拟与污染控制国家重点联合实验室专项基金课题(No. 10Y04ESPCT,2010 ~ 2012):活性病原菌选择性检测技术及在水处理过程中的去除特性研究,主要完成人,已完成。
[12]环境模拟与污染控制国家重点联合实验室专项基金课题(No. 08Y01ESPCT,2008 ~ 2010):水中特征病原微生物高通量快速检测技术研究开发,主要完成人,已完成。

 

讲授课程:

本科生课程:环境毒理学原理、环境科学前沿

研究生课程:环境毒理学

 

代表性论文:

[1]   Zhang Y., He M., Li D*. Sub-inhibitory Concentrations of Heavy Metal Ions Accelerate the Horizontal Transfer of Plasmid-Mediated Multidrug Resistance Genes. Environmental Science& Technology. (Submitted)

[2]   Zhang Y., Gu A. Z., He M., Li D*. Subinhibitory Concentrations of Disinfectants Promote the Horizontal Transfer of Multidrug Resistance Genes within and across Genera. Environmental Science& Technology. DOI: 10.1021/acs.est.6b03132.

[3]   D. Li, S. Zeng, M. He* & A. Z. Gu*. Water Disinfection Byproducts Select for Antibiotic Resistance-Role of Environmental Pollutants in Resistance Phenomena. Environmental Science& Technology. 2016, 50, 3193−3201.

[4]   Lin Y.W., Li D.,* Zeng S. Y., He M.* Changes of microbial and pathogenic compositions during wastewater reclamation and distribution systems revealed by high-throughput sequencing analyses. Frontiers of Environmental Science and Engineering. June 2016, Volume 10, Issue 3, pp 539–547

[5]   Lin Y.W., Li D.,* Gu A. Z., He M.* Bacterial regrowth in water reclamation and distribution systems revealed by viable bacterial detection assays. Chemosphere, 2016, 144, 2165-2174.

[6]   Siyu Zeng; Yunqing Huang; Fu Sun; Dan Li; Miao He. Probabilistic Ecological Risk Assessment of Effluent Toxicity of a Wastewater Reclamation Plant Based on Process Modeling. Water Research, 2016, 100, 367-376.

[7]   Rui Li, Cen Zhao, Bo Yao, Dan Li, Shuwen Yan, Kevin E. O’Shea, and Weihua Song. Photochemical Transformation of Aminoglycoside Antibiotics in Simulated Natural Waters. Environmental Science& Technology. 2016, 50 (6), pp 2921–2930

[8]   Wang Y.Z., Li D., He M.* Application of internal standard method in recombinant luminescent bacteria test. Journal of Environmental Sciences 2015, 35, 128 – 134.

[9]   Dong X., Zeng S., Bai F., Li D., He M. Extracellular microcystin prediction based on toxigenic Microcystis detection in a eutrophic lake. Scientific Report. 2016, 6:20886. DOI: 10.1038/srep20886

[10]         Li D.*, Tiezheng Tong, Siyu Zeng, Yiwen Lin, Shuxu Wu1, Miao He*. Quantification of viable bacteria in wastewater treatment plants by using propidium monoazide combined with quantitative PCR (PMA-qPCR). Journal of Environment Science. 2014,(26) 299–306.

[11]         Li D., A. Z. Gu and M. He. Quantification and genetic diversity of total and microcystin-producing Microcystis during blooming season in Tai and Yang-cheng lakes, China. Journal of Applied Microbiology. 2014, 116, 1482-1494.

[12]         Li, D., S.X. Wu, M. He*, H.C. Shi. Inactivation, Reactivation and Regrowth of Indigenous Bacteria in Reclaimed Water after Chlorine Disinfection of a Municipal Wastewater Treatment Plant. Journal of Environment Science. 2013, 25(7): 1319-1325.

[13]         Li, D., A. Z. Gu, W. Yang, M. He*, and H. C. Shi. Monitoring and Evaluation of Infectious Rotaviruses in Various Wastewater Effluents and Receiving Waters Revealed Correlation and Seasonal Pattern of Occurrences. Journal of Applied Microbiology. 2011, 110: 1129-1137.

[14]         W. Yang., A. Z. Gu, D. Li *, M. He*, and H. C. Shi. Development of a Combined Immunomagnetic Separation and Quantitative Reverse Transcription-PCR Assay for Sensitive Detection of Infectious Rotavirus in Water Samples. Journal of Microbiological Methods 2011, 84: 447–453.

[15]         Li, D., A. Z. Gu, W. Yang., M. He*, and H. C. Shi. Evaluation of the infectivity, genome and antigenicity persistence of rotavirus after chlorine disinfection. Journal of Environmental Science. 2011, 23(10): 1691–1698.

[16]         Li, D., M. He and S. Jiang*. Detection of infectious adenoviruses in environmental waters by fluorescence-activated cell sorting assay. Applied and Environmental Microbiology. 2010, 76: 1442–1448.

[17]         Li, D., H. C. Shi., and S. Jiang*. Concentration of Viruses from Environmental Waters using Nanoalumina Fiber Filters. Journal of Microbiological Methods 2010, 81: 33–38.

[18]         Li, D., A. Z. Gu, W. Yang, M. He*, and H. C. Shi. Rapid and sensitive detection of infectious rotaviruses in environmental water by an integrated cell culture and real-time RT-PCR assay. Journal of Microbiological Methods 2010, 82: 59–63.

[19]         Li, D., A. Z. Gu, M. He*, H. C. Shi, and W. Yang. UV inactivation and resistance of rotavirus evaluated by integrated cell culture and real-time RT-PCR assay. Water Research. 2009, 43: 3261-3269.

国际会议:

[1]     Zhang Y., Huang X.X., Li D*. Sub-lethal levels of disinfectants promote the horizontal transfer of multidrug resistance genes between and across genera. The 4th International Conference on Environmental Simulation and Pollution Control, Beijing, November 2-3, 2015. (Best Oral Prize)

[2]     Lou W.H., Huang X.X., Li D*. Modeling mixture genotoxicity by recombined bacterial biosensors specific for SOS response. The 4th International Conference on Environmental Simulation and Pollution Control, Beijing, November 2-3, 2015.

[3]     Cen T., Zhang Y., Li D*. Three Preservatives Promote the Transfer Frequency of Antibiotic Resistance Genes Mediated by Plasmid pCM184. The 4th International Conference on Environmental Simulation and Pollution Control, Beijing, November 2-3, 2015.

[4]     Li D., M. He, H.C. Shi. Rapid and sensitive detection of infectious rotaviruses in environmental water by an integrated cell culture and RT-PCR (ICC-RT-PCR). 1st Proceedings of International Workshop on Monitoring and Sensor for Water Pollution Control. Beijing: 2007. 117-129.

[5]     Li D., He M., Shi H. An integrated cell culture and quantitative PCR assay for determining infectious rotavirus in environmental waters. 2nd Proceedings of International Workshop on Monitoring and Sensor for Water Pollution Control. Beijing: 2009. 114-119.

[6]     Li D., S.X. Wu, M. He, H.C. Shi. Inactivation, Reactivation and Re-growth of Indigenous Bacteria in Reclaimed Water after Chlorine Disinfection of a Municipal Wastewater Treatment Plant. 2nd Water Research Conference. Singapore: 01/21/2013 ~ 01/23/2013.

[7]     Lin Y.W., D. Li,* S.X. Wu, M. He. Development of Reverse Transcription Quantitative PCR assay for Detection and Quantification of Viable Pathogenic Bacteria in waters. Proceedings of International Conference on Environment Simulation and Pollution Control. Beijing: 2011.

[8]     Yang T., D. Li,* M. He. Selectively detection of infectious pathogenic bacteria in environmental waters by fluorescent staining combined with flow cytometry. Proceedings of International Conference on Environment Simulation and Pollution Control. Beijing: 2011.

[9]     Wu S.X., D. Li,* M. He. Transcriptomic analysis of the responses of Escherichia coli to chlorine-based oxidative stress. Proceedings of International Conference on Environment Simulation and Pollution Control. Beijing: 2011.

申请专利:

[1]   李丹,何苗,施汉昌,刘丽. 一种定量检测微囊藻的方法及其专用标准品. 授权号:201010525990.2

[2]   李丹,施汉昌,何苗,刘丽. 一种定量检测产毒微囊藻的方法及其专用标准品.授权号:201010525975.8

[3]   李丹,林怡雯,何苗. 用于检测分枝杆菌的引物对和标准品以及它们的应用.公开号:201310031783.5

[4]   何苗,李丹,林怡雯. 一种检测活性细菌含量的方法. 公开号:201210438843.0

[5]   何苗,李丹,施汉昌,杨万,胡昕. 一种浓集污水和污水处理厂尾水中病毒的方法. 授权号:ZL 200710175738.1.

[6]   何苗,林怡雯,李丹. 定量检测大肠杆菌RNA的方法及其专用标准品和应用.公开号:201210202181.7

[7]   何苗,胡秀华,刘丽,李丹,施汉昌. 一种定量检测轮状病毒的方法及其专用标准品. 授权号:ZL 200710099584.2.

 

 

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